Terminator

Part:BBa_K2680534

Designed by: Julia Urban   Group: iGEM18_William_and_Mary   (2018-10-13)


Terminator.png

T500_no Gap, short attachment (T11) terminator

  • Variant of T500 terminator.

T500 is a strong intrinsic terminator "derived from bacteriophage Φ82".1,2 Requires hypertranslocation by RNA polymerase.2

References

1Shin, J., & Noireaux, V. (2010). Efficient cell-free expression with the endogenous E. Coli RNA polymerase and sigma factor 70. Journal of Biological Engineering, 4(1), 8. doi:10.1186/1754-1611-4-8

2Greenblatt, Jack. F. (2008). Preview: Transcription termination: pulling out al the stops. Cell, 132, 918. doi:10.1016/j.cell.2008.03.003 Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Measurement

  • [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]
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Categories
Parameters
None